Phenol 0 polymerase inhibition/run failureĬhloroform 0 polymerase inhibition/run failureĬsCl 0.4mM polymerase inhibition/run failureĮDTA/salts 0.2mM injection failure/weak signal II. NaOAc 0.4mM polymerase inhibition/run failure PEG 0.3% polymerase inhibition/run failureĮtOH 1.2% polymerase inhibition/run failure Note that the following contaminants can negatively impact sequencing.Ĭontaminant Max level of tolerance Effect These silica membrane products adsorb high salt contaminants producing DNA templates in water or low-salt buffer. There is also Promega Wizard and many other products including Fisher (Life Technologies) GenJet PCR Purification or Zymo Research to among others. Methods for purification of high-quality templates include QIAprep products (from QIAGEN) which include individual columns, strips of 8, or 96-well plates. Sanger (BigDye) Sequencing Services Protocols I. Facility Rates can be found via the IIGB Billing and Reservation site.
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